Impaired Skeletal Muscle Regeneration in the Absence of Fibrosis during Hibernation in 13-Lined Ground Squirrels
Figure 2
Satellite cells are not involved in maintenance of skeletal muscle during hibernation.
Irradiation was used to ablate satellite cells from one quadriceps muscle of hibernating squirrels. A: Hematoxylin-eosin (H&E) staining revealed no morphological differences between untreated control (left) and irradiated (right) quadriceps. B: Dystrophin immunolabeling outlined myofiber sarcolemmas enabling determination of percentage distribution of minimal Feret's diameter and revealed no effect of satellite cell ablation (scale bar 100 µm). C: Mean minimal Feret's diameter (µm) was not significantly different between control and irradiated quadriceps during hibernation (n = 3).