DNA Repair and Cell Cycle Biomarkers of Radiation Exposure and Inflammation Stress in Human Blood
Figure 6
LPS mediated suppression of phosphorylated CHK2-thr68 protein at 24 hrs after 2 Gy exposures.
Protein levels of phosphorylated CHK2-thr68 in protein lysate from cultured whole blood in the presence or absence of LPS (50 ng/ml) were measured by ELISA. Absorbance values were normalized with respect to the average pCHK2-thr68 level in unirradiated donors. In the absence of LPS, radiation induced CHK2-thr68 levels ∼1.6-fold (±0.1) relative to sham irradiated samples, whereas in the presence of LPS, CHK2-thr68 levels were indistinguishable from sham irradiated samples (p>0.4).