CD69 Does Not Affect the Extent of T Cell Priming
Figure 6
CD69 targeting does not alter CD8+ T cell priming threshold at different peptide agonistic affinities.
Whole LN and spleen OT-I RAG1−/− cells were stained with 2 µM CFSE and cultured at 106 cells per well with the indicated doses of SIINFEKL (A), SIIGFEKL (B), Catnβ1 (C) and Catnβ1 plus 1 µg/ml of LPS (D), and 10 µg/ml of anti-CD69 2.2 mAb (dashed line) or isotype control (solid line). Graphs showing the number of proliferated (left column) and of CD25+ (right column) CD8+ T cells per well. Results representative of two experiments.