Expression of Small RNA in Aphis gossypii and Its Potential Role in the Resistance Interaction with Melon
Figure 5
In vitro feeding assay with A. gossypii.
A. Sachet feeding system. B. Honeydew droplets collected on a blotting sheet viewed by phosphor-imager C. 15% PAGE analysis of RNA and honeydew isolated from the aphids feeding on labeled diet and exposed to autoradiograph. Lane 1–2, Total RNA isolated from aphids; 3–4, honeydew elutions; 5, positive control ([γ-32P]-ATP labeled ds-21 nt).