Transforming Growth Factor Alpha (TGFα) Regulates Granulosa Cell Tumor (GCT) Cell Proliferation and Migration through Activation of Multiple Pathways
Figure 3
Effect of TGFα on KGN cell proliferation.
A) KGN cells were treated without (CTL) or with TGFα (10 ng/ml) in DMEM supplied with increasing amounts of serum for 48 hours and cell numbers were counted. B) KGN cells were treated without (Control) or with TGFα and/or different kinase inhibitors in DMEM for 48 hours and cell numbers were counted. Bars represented means ± SEM, Bars with different letters are significantly different (P<0.05) from each other. CTL = control; AG1478 (100 nM): EGFR kinase inhibitor; U0126 (4 µM): MEK inhibitor; Rapamycin (20 nM): mTOR inhibitor; LY294002 (100 nM), PI3K inhibitor.