Single Cell Analysis of Yeast Replicative Aging Using a New Generation of Microfluidic Device
Figure 6
Age dependent asymmetric partitioning of a stress response reporter between mother and daughter cells.
(a) The sizes of each mother–daughter pair for mother cells at different time after initial loading. As mother cell size grows with time, the size of the daughter only increase slightly. (b) box plot of the mother-daughter size ratio for the pairs measured in (a). There is a significant increase of the ratio over time (*PT-test = 5.5×10−6, **PT-test = 2.4×10−5). (c) The ratio of the stress response reporter level in the mother cell vs. that in its daughter. Each point represents a mother-daughter pair. Time is relative to the initial loading of the mother cells. (d) box plot of data in (c). There is a significant difference between time point 1 and 2, and point 2 and 3 (*PT-test = 4.0×10−2, **PT-test = 2.5×10−2).