One-Step Agrobacterium Mediated Transformation of Eight Genes Essential for Rhizobium Symbiotic Signaling Using the Novel Binary Vector System pHUGE
Figure 6
MtNIN induction upon Rhizobium application in legumes and non-legumes.
Induction of MtNIN 24 h after exposure to flavonoid stimulated, compatible rhizobium bacteria (+) compared to water control (−) in Medicago (Med WT), transgenic poplar (line Pop 15.4 and Pop 18.1) and transgenic tobacco (line Tob 47 and Tob 65). Poplar PtACT2 & PtUBQ and tobacco NtGAPDH were used as reference genes. Three biological replicates are shown for each line. Error bars show variation between three technical replicates.