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Routine Habitat Change: A Source of Unrecognized Transient Alteration of Intestinal Microbiota in Laboratory Mice

Figure 6

Eubacterial-TRFLP analysis of fecal microbiota in mice following two routine cage changes.

Taxonomic profile (left) and principal coordinate analysis (right) of TRFLP data. Operational taxonomic units were assigned based on an in silico digest-database to estimate relative proportions of each major bacterial group present in the samples. Each bar represents a single mouse specimen prior to cage change (d7, d21), 1 day after cage change (d8, d22), and 5 days after cage change (d12, d26). Each PCoA data point represents these individual specimens prior to cage change (yellow squares, d7, d21), 1 day after cage change (black circles, d8, d22), and 5 days after cage change (blue triangles, d12, d26). Mice X, Y, Bb, and Cc were males; W, Z, and Aa were females.

Figure 6

doi: https://doi.org/10.1371/journal.pone.0047416.g006