Towards Defining Nutrient Conditions Encountered by the Rice Blast Fungus during Host Infection
Figure 3
Deleting MoSTR3 from the M. oryzae genome results in a strict requirement for exogenous methionine.
(A) Growth of Δstr3 strains was tested on 1% glucose minimal media (GMM) with the indicated sole nitrogen sources added to a final concentration of 10 mM. As predicted from Figure 1, Δstr3 strains cannot grow on minimal media unless methionine is used as the sole nitrogen source. (B) If methionine is supplied in the media (as sole nitrogen source at 10 mM concentration), Δstr3 strains (grey bar) sporulate like wild type Guy11 (closed bar). Spore counts were determined following 14 days of growth using 5 independent replicates for each strain. Bars with the same letters are not significantly different (Student's t-test p≤0.05). Error bars are standard deviation. (C) Transforming methionine-requiring Δstr3 strains with a wild type copy of MoSTR3 complements the Δstr3 genetic lesion and allows growth of Δstr3 MoSTR3 strains on minimal media lacking methionine. All plate images were taken after 10 days growth.