Development of Bioluminescent Bioreporters for In Vitro and In Vivo Tracking of Yersinia pestis
Figure 6
Dissemination of Y. pestis during bubonic infection.
Mice were infected with ∼200 CFU of Y. pestis LuxPcysZK subcutaneously at the base of the tail (A) or in the footpad (B) and imaged using an IVIS Spectrum. The lymph node drainage basin for each inoculation site is diagrammed above the images [24], [25]. Location of the inoculation site is shown as a green circle, lymph nodes as blue circles, and the spleen as a red oval. For (A), the white arrow denotes the subiliac LN and the red arrow the axillary LN. For (B), the white arrow denotes the popliteal LN, the red arrow the sciatic LN, and the yellow arrow the renal LN. All images were adjusted to the radiance scale shown, except for the images in (B) marked with * in upper right corners. For these each image was adjusted to a different radiance to allow for visualization of specific tissues.