Novel Nongenomic Signaling by Glucocorticoid May Involve Changes to Liver Membrane Order in Rainbow Trout
Figure 1
Cortisol effect on plasma membrane order in vitro.
A) 1,6-Diphenyl-1,3,5-hexatriene (DPH) fluorescence anisotropy of enriched hepatic plasma membranes isolated from rainbow trout treated with or without benzyl alcohol for 30 min prior to anisotropy measurement at various temperatures. Data represents mean ± S.E.M (n = 7 independent fish). Different upper case letters indicate significant temperature effects and inset indicates significant treatment effects (two-way repeated measures ANOVA, p<0.05). B) DPH fluorescence anisotropy of enriched hepatic plasma membrane fractions treated with cortisol (0, 10, 100, 500, and 1000 ng/ml) for 30 min at various temperatures. Data represents mean ± S.E.M (n = 6 independent fish). Different upper case letters indicate significant temperature effects and inset indicates significant treatment effects (two-way repeated measures ANOVA, p<0.05). C) DPH fluorescence anisotropy of isolated hepatic plasma membrane fractions treated with the peptide conjugate (PEP, equivalent to 1000 ng/ml, 2.75 µM), or cortisol-conjugated peptide (cortisol-PEP, 0.275 µM and 2.75 µM) for 30 min prior to anisotropy measurement at various temperatures. Data represents mean ± S.E.M (n = 5 independent fish). Different upper case letters indicate significant temperature effects (one-way repeated measures ANOVA, p<0.05). D) DPH fluorescence anisotropy of enriched trout hepatic plasma membrane fractions treated with 17β-estradiol (E; 1 µM) or testosterone (T; 1 µM) for 30 min. Data shown as mean ± S.E.M (n = 6 independent fish).