The Effects of Amino Acid Composition of Glutamine-Rich Domains on Amyloid Formation and Fragmentation
Figure 1
Polymerization of polyQX proteins in the presence of [PIN+].
Lysates of 74-D694/ΔS35 [PIN+] cells producing indicated QX proteins were analyzed by SDD-AGE. (A) Most QX proteins readily form SDS-insoluble polymers of varying size. Staining with anti-S35NM antibodies. (B) Polymerization of QX proteins, which did not polymerize in a [PIN+] background on their own, in the presence of 85Q-GFP polymers and after loss of the plasmid encoding 85Q-GFP. Staining with anti-Sup35C.