Paralogous ALT1 and ALT2 Retention and Diversification Have Generated Catalytically Active and Inactive Aminotransferases in Saccharomyces cerevisiae
Figure 3
ALT1 and ALT2 display differential gene expression pattern, which is consistent with Alt1-Alt2 intracellular concentration.
Northern blot of total RNA prepared from wild type strain grown on either glucose-ammonium (A) or glucose-alanine (B). Samples were collected from various OD600 as stated. Representative results from three experiments are shown. Cell-free extracts from ALT1-TAP (C and E) or ALT2-TAP (D and F) tagged strains grown on glucose-ammonium (C and D) or glucose-alanine (E and F) were prepared and subjected to immunoblot analysis using anti-TAP polyclonal antibody. As a loading control, each nitrocellulose membrane was also subjected to immunoblot analysis using anti-Lys20/Lys21 antibody. All lanes were loaded with 100 µg of protein. An ALT1-TAP sample was included as a loading control in panel F. Results are representative of three independent experiments.