Transient Scrotal Hyperthermia Induces Lipid Droplet Accumulation and Reveals a Different ADFP Expression Pattern between the Testes and Liver in Mice
Figure 5
The trapping cassette of AdfpΔ1/Δ1 mice is inserted in the intron between exons 1 and 2, before the Adfp translation start site.
The peroxisome proliferator-activated receptor responsive element (PPRE) is located ∼2,000 bp upstream of the transcription start site (A). RT-PCR products are presented using primers targeted to exons 1–3, exons 3–8, and exons 6–8 of the Adfp transcripts of testes and liver from AdfpΔ1/Δ1 mice and wild type mice. β-actin was amplified as the control gene (B).