A Lateral Flow Assay for Quantitative Detection of Amplified HIV-1 RNA
Figure 4
NASBA was performed using 0, 5, 50, 500, or 5000 copies of in vitro transcribed HIV gag RNA as a template. Products were diluted by a factor of 10, 100, and 1000, and a 20 µL volume of each dilution was dispensed onto a lateral flow strip for detection. (A) Scanned image of strips for each concentration of NASBA products and dilution factor. Note that although the contrast was adjusted in the figure, raw images were used for signal-to-background calculations. (B) Dose response curve constructed using the signal-to-background ratio of 100-fold diluted products. The negative control SBR is shown for comparison.