High-Resolution Transcriptome of Human Macrophages
Figure 5
Network analysis of RNA-seq data.
(A) Network of genes highly expressed in M1-like macrophages (fold-change >4.0) identified by RNA-seq. (B) Data generated by microarray analysis were loaded into the M1-network established using RNA-seq. (C) Network of genes highly expressed in M2-like macrophages (fold-change >2.5) identified by RNA-seq. (D) Data generated by microarray analysis were loaded into the M2-network established using RNA-seq. All networks were generated using EGAN. (E) APOL3 and (F) LILRB1 expression in human M1- and M2-like macrophages. Far left, relative expression as determined by RNA-seq. Left, representative images of sequencing reads across genes expressed in human macrophages as described in Fig. 4. Right, relative mRNA expression by qPCR in M1- and M2-like macrophages. Far right, protein data as determined by immunoblotting, respective flow cytometry. Data are representative of three experiments (RNA-seq, qPCR, and immunoblotting resp. flow cytometry; mean and s.e.m.) each with cells derived from a different donor. Isotype controls are depicted as dotted lines. *P<0.05 (Student’s t-test).