Loss of the V-ATPase B1 Subunit Isoform Expressed in Non-Neuronal Cells of the Mouse Olfactory Epithelium Impairs Olfactory Function
Figure 4
Electron micrographs of the olfactory epithelium from a 2-week old female mouse pup.
The V-ATPase A subunit (panel A, arrowheads) is expressed at high levels in the microvilli of sustentacular cells (SC), but is minimally present in the olfactory sensory neurons (OSN), where it localizes to the cytosolic domain. Panel B shows the same expression pattern for the V-ATPase (arrowheads, 15 nm gold particles), whereas tubulin (arrows, 10 nm gold particles) localization is restricted to OSN cilia (shown at higher magnification in inset). Bar = 0.5 µm. Inset bar = 0.1 µm.