Model Hirano Bodies Protect against Tau-Independent and Tau-Dependent Cell Death Initiated by the Amyloid Precursor Protein Intracellular Domain
Figure 10
Model Hirano bodies lower tau-dependent and tau-independent apoptosis.
H4 cells were transfected with either APP/c31 or APP/c31/352PHP/tau in the absence (GFP-black bars) or presence of model Hirano bodies (CT-GFP-check bars). H4 cells were stained with the early apoptotic marker annexin V. Sytox orange was used as a control for annexin V false positive cells with compromised membranes. Cells transfected with either GFP/APP/c31 (tau-independent) or GFP/APP/c31/352PHPtau (tau-dependent) showed significant levels of annexin V positive staining. The presence of model Hirano bodies lowered the percent of cells that were annexin V positive in both tau-independent and -dependent cell death (* p<0.05 in comparison to cells without model Hirano bodies). Error bars represent the standard deviation.