Expression of MUC17 Is Regulated by HIF1α-Mediated Hypoxic Responses and Requires a Methylation-Free Hypoxia Responsible Element in Pancreatic Cancer
Figure 4
Methylation status of HRE within the MUC17 proximal promoter region.
(A) Differential expression pattern of MUC17 induced by hypoxic exposure in pancreatic cancer cell lines. Each pancreatic cell line was cultured under normoxic (N) or hypoxic (H) conditions. Level of mRNA was determined by RT-PCR at each time point. (B) The human MUC17 gene promoter sequence, which spans positions −687 to +302 with respect to the transcription start site. The numbers of the CpG sites are underlined. The HRE site contains the CpG site (No. 13).