Depletion of the C. elegans NAC Engages the Unfolded Protein Response, Resulting in Increased Chaperone Expression and Apoptosis
Figure 1
Mortality and movement of ICD-1 or ICD-2-depleted animals during heat stress.
Developmentally synchronized wild-type (N2) C. elegans larvae were fed icd-1 or icd-2(RNAi)-specific bacteria for 30 hours and exposed to continuous heat stress at 36°C for 850 minutes. A) Survival of animals was measured by periodic assessment of pharyngeal pumping. B) Movement was measured by periodic assessment of escape reaction to physical prodding. N = 50 for each of the three populations tested per experiment. Data presented in A) and B) represent the results of two independent trials for each experiment. The three experimental populations were also exposed to no heat (20°C) as a measure of basal rates of mortality and effects on movement, and showed no inherent lethality or immobility (N = 50 per population) (data not shown).