Pseudotyped AAV Vector-Mediated Gene Transfer in a Human Fetal Trachea Xenograft Model: Implications for In Utero Gene Therapy for Cystic Fibrosis
Figure 4
Immunostaining with heparan sulfate proteoglycan (HSPG), the AAV2 receptor on fetal and adult tracheas.
Immunostaining at 10× and 40× magnification in fetal (A and B) and adult trachea (C and D) respectively. Expression of HSPG was limited to the basolateral surface of the respiratory epithelium (arrows). There is no staining of the airway epithelium (arrow heads) in either model. Luminal surface of the trachea (L).