The Human Milk Protein-Lipid Complex HAMLET Sensitizes Bacterial Pathogens to Traditional Antimicrobial Agents
Figure 5
Impact of HAMLET on uptake and binding of gentamicin and Bocillin FL.
(A) S. pneumoniae D39 were incubated with Alexa Fluor 488-gentamicin in the presence or absence of HAMLET. HAMLET significantly increased the cell-associated level of gentamicin. (B) The penicillin-resistant strain SP670 and the penicillin-sensitive strain D39 were incubated with the fluorescent beta-lactam Bocillin FL in the presence or absence of HAMLET. HAMLET did not increase the cell-associated level of Bocillin FL in either strain. The results are based on three individual experiments with duplicate samples. Statistics was performed using the unpaired Student t-test. Significance was indicated as follows: ** = P<0.01, *** = P<0.001, ns = not significant.