Multiphoton Multispectral Fluorescence Lifetime Tomography for the Evaluation of Basal Cell Carcinomas
Figure 1
Multispectral fluorescence intensity and FLIM images acquired from normal skin and BCCs.
(a–i) Fluorescence intensity and false color FLIM images from a single field of view acquired at a depth of 110 µm with all spectral channels taken near a dermal papilla from normal skin. (j–n) FLIM images taken from the green channel only of different depths within a sample of normal skin. (o–u) FLIM images taken from the green channel illustrating visual architectural features seen in BCC using MPT. (v) FLIM image taken from the blue channel of a BCC. (w,x) paired FLIM images taken from the green and blue channels respectively of a BCC nest. KEY SG-Stratum Granulosum, SS- Stratum Spinosum, BCL-Basal Cell Layer, DP-Dermal Papilla. Scale bar 25 µm.