Growth Factor-Activated Stem Cell Circuits and Stromal Signals Cooperatively Accelerate Non-Integrated iPSC Reprogramming of Human Myeloid Progenitors
Figure 3
Endogenous expression of chromatin remodeling factors known to augment reprogramming efficiency in parental donor cells.
Relative expressions of genes in chromatin remodeling factors in parental donor samples. (a) MYC complex regulators, (b) PRC2 complex regulators, (c) Trithorax complex regulators, (d) SWI/SWF family regulators, and (e) Chromodomain (CHD) regulators in day 0 GF-activated CB; pooled CB donors; n = 3 samples), adult fibs (adFibs; n = 3 samples), and hESC (n = 5 samples). Heatmaps and adjacent boxplots show log2 mean-subtracted normalized values of signal intensities from averaged, independent biological replicate microarray samples (n = 3–5 per condition) averages of values, with *p<0.05 where indicated. In mean normalization, each gene’s mean log2 signal value is determined for all the cell types, and then subtracted from each cell type’s signal intensity value for that gene.