A Key Role for the Endothelium in NOD1 Mediated Vascular Inflammation: Comparison to TLR4 Responses
Figure 1
Induction of iNOS activity by NOD1 and TLR4 ligands in whole rat aorta.
Aortic rings with endothelium intact (+EC) and denuded (−EC) were treated for 24 hours with media alone (CTRL), C12-iE-DAP 1 µg/ml (NOD1 agonist) or LPS 1 µg/ml (TLR4 agonist) (A) In some experiments, cells were treated for a further 24 hour period with replacement of media and agonists (48 hours; B). In addition, endothelium intact rings were pre-treated with the iNOS inhibitor 1400 W 1 µM (C) or the IκBβ inhibitor SC-514 30 µM (D) for 30 minutes prior to 24 hours treatment with agonists. iNOS activity was assessed by measurement of nitrite (breakdown product of nitric oxide) by the Griess assay. Results are expressed as mean ± SEM for n = 9 (A+B) or n = 4 (C+D) animals. Data was analysed by one-way ANOVA followed by Bonferroni’s Multiple Comparison Test (*P<0.05) or by two-way ANOVA followed by Bonferroni’s post tests (+P<0.05).