The Alveolin IMC1h Is Required for Normal Ookinete and Sporozoite Motility Behaviour and Host Colonisation in Plasmodium berghei
Figure 5
Characteristics of sporozoite motility in Matrigel.
A. Speed of WT (n = 15) and IMC1h-KO (n = 11) sporozoites. B. Results of the manual assignment of movement patterns of WT (n = 15) and IMC1h-KO (n = 11) sporozoites. M = Meandering, CW = clock-wise, and CCW = counter clock-wise. C. Reconstruction of tracked sporozoite paths from high-magnification movies revealing movement patterns (two frames per second for 100 s). WT sporozoites (upper panel) mainly display a circular mode of motility while IMC1h-KO sporozoites (lower panel) mainly show a meandering mode of motility. Scale bar = 20 µm. D. Brightfield time-lapse image sequences showing motile WT and IMC1h-KO sporozoites. Scale bar = 10 µm.