Loss of Activating EGFR Mutant Gene Contributes to Acquired Resistance to EGFR Tyrosine Kinase Inhibitors in Lung Cancer Cells
Figure 2
Comparison of EGFR gene and protein expression between PC9 and PC9/ER1 cells.
A, Western blots showing expression of delE746-A750 EGFR and total EGFR in PC9 and PC9/ER1 cells. B, Detection of wild-type and mutation sequences using specific primers. PC9 cells show both wild-type and mutant-specific bands, while PC9/ER1, 11–18, and QG56 cells show only the wild-type-specific band. Mut, mutant exon19, and WT, wild-type exon19. C, PCR analysis shows heteroduplex (Mut/WT) and homoduplex (WT/WT and Mut/Mut) in PC9 cells, and only homoduplex (WT/WT) in PC9/ER1 cells, 11–18 cells harboring L858R mutation, and QG56 cells harboring wild-type EGFR.