Differentially Methylated Regions of Imprinted Genes in Prenatal, Perinatal and Postnatal Human Tissues
Figure 2
Pyrosequencing validation using bisulfite modified methylated and unmethylated IGF2 and H19 DMR sequences in plasmids.
Plasmids were quantified and mixed by pipetting to generate specimens containing 5% incremental increases in methylated DNA over the full range of possible methylation values (0% to 100%). Error bars, SD for duplicate measurements.