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Similarities of Drosophila rab GTPases Based on Expression Profiling: Completion and Analysis of the rab-Gal4 Kit

Figure 1

Targeting vector design for rab30-Gal4, rab40-Gal4, rabX5-Gal4 and rabX6-Gal4.

20–22 kb genomic regions (black bars) were recombineered from bacterial artificial chromosomes (BACs) into attB-P[acman]-KO [17], [22]. Regions of a few kb are shown at higher resolution to reveal the structures of rab loci within these genomic regions. Sequences between red arrows were replaces with a Gal4 knock-in cassette [17]. For expression analyses, transgenic flies with the targeting vectors inserted in the same landing site were used.

Figure 1

doi: https://doi.org/10.1371/journal.pone.0040912.g001