High Passage MIN6 Cells Have Impaired Insulin Secretion with Impaired Glucose and Lipid Oxidation
Figure 5
Gene expression in HP and LP MIN6 cells.
A) HP MIN6 cells had increased expression of Pdx1 and reduced expression of proinsulin. B) HP MIN6 cells had reduced expressions of the glucose transporter Glut1 and the key glycolytic gene Pfk. There was a greater than 2-fold increase in the glycolytic gene Ldh-A in high passage MIN6 cells. C) HP MIN6 cells had increased expressions of Hk1 and Hk2, accompanied with a decrease in Gck. D) HP MIN6 cells also had reduced expressions of cholesterol synthesizing genes HMG CoA reductase and HMG CoA synthase, and the transcription factor for lipid synthesis Srebp1C. E) HP MIN6 cells had increased expression of lipid oxidation genes Acadm, Ehhadh, Acaa1a and increased Hadh expression. F) HP MIN6 cells had reduced Ucp2, Sirt3, and Nampt expression. Error bars are ± SEM and n = 3.