Mouse-Adapted H9N2 Influenza A Virus PB2 Protein M147L and E627K Mutations Are Critical for High Virulence
Figure 1
Viral growth kinetics of SD16 and SD16-MA in MDCK and A549 cells.
Confluent MDCK (A) or A549 (B) cells were infected with H9N2 viruses at an MOI of 0.01. Virus yields at 12, 24, 36, 48, and 60 hpi were titrated in MDCK cells. Each data point represents the mean virus yield from three individually infected wells ± SD.