Pulmonary Infection with Influenza A Virus Induces Site-Specific Germinal Center and T Follicular Helper Cell Responses
Figure 6
Splenectomized mice withstand a secondary 10.0LD50 IAV infection.
Sham or splenectomized animals were given a sublethal 0.1LD50 dose of IAV i.n. on day 0 and a subsequent 10.0LD50 dose of IAV i.n. on day 42. Morbidity and mortality were monitored through day 50. Serum samples were harvested on day 28 following primary sublethal infection. A) Line graphs represent percent of starting body weight (morbidity) after primary and secondary infection. B) Line graphs represent survival (mortality) after primary and secondary infection. C) IgG1, IgG2a, IgG2b and IgG3 Ab responses were measured in day 28 post-infection sera using an IAV-specific whole virus ELISA. Sera from naïve mice were also tested. Each value represents mean ± SEM. n = 4 for sham splenectomized mice. n = 5 for splenectomized mice. *p<0.05; represents the statistical difference between sham and splenectomized mouse sera at the indicated dilutions as determined by the unpaired Student's t test.