Mouse Transgenesis in a Single Locus with Independent Regulation for Multiple Fluorophores
Figure 2
Triple transgenic mouse line targets three major cell types of the brain.
A–C) Expression of three transgenes from Prism JD1849 mouse line is largely similar to patterns of single transgenic lines; A) Snap25::mCherry, B) Aldh1L1::Cerulean-Myc C) Mobp::YFP-Ha. D) Overlay of A–C. Box shows approximate location of E. E) Single panel showing division (dashed line) between white matter of corpus callosum (containing only oligodendrocytes and astrocytes) and gray matter of cortex (containing all three cell types). F, G) Confocal microscopy of cortex (F) and cerebellum (G) shows distinct morphology for each cell type, and lack of overlap for fluorophores. Nuclei counterstained with To-Pro-3-iodide (white).