Loss of DJ-1 Does Not Affect Mitochondrial Respiration but Increases ROS Production and Mitochondrial Permeability Transition Pore Opening
Figure 3
Decreased ATP concentration in DJ-1−/− MEFs.
(A, B) Western analysis of each subunit in the oxidative phosphorylation (OXPHOS) complex in DJ-1−/− and +/+ MEFs. (A) Representative western blot showing relative expression of each subunit. Tubulin was used as loading control. Non-specific bands are marked by asterisk. (B) The bar graph shows the quantification and normalization of the expression level of each subunit using tubulin as loading control. (C) Enzymatic activities of complexes I, II and IV of the mitochondrial electron transport system, as measured by spectrophotometric assays and after normalization to citrate synthase activity (CS). (D) The bar graph shows decreased ATP concentrations in DJ-1−/− MEFs compared to control cells. The number shown in the panel indicates the number of embryos used to derive primary MEFs per genotype, and the data were obtained from three independent experiments. All data are expressed as mean ± SEM. *p<0.05.