Insight into Temperature Dependence of GTPase Activity in Human Guanylate Binding Protein-1
Figure 9
▵GD of wild type and mutant hGBP1 in the absence and presence of GppNHp.
Heat-induced denaturation studies of wild type and mutant proteins were carried out with or without GppNHp. The experimental data were fitted using Eq. 2 to obtain ▵Hm and Tm. ▵Cp of the mutant proteins was assumed to be same as in wild type. With these values, the ▵GD at 37 °C was calculated using Eq. 3.