The Effects of Promoter Methylation on Downregulation of DAZAP2 in Multiple Myeloma Cell Lines
Figure 4
The activity analysis of promoter region upstream of DAZAP2 gene.
The amplified DNA fragments in DAZAP2 promoter region were inserted to luciferase reporter gene vector pGL2-Basic and luciferase activity was detected. The empty vector pGL2-Basic was used as negative control. The vector pGL2-Control, containing SV40 promoter and enhancer, can express luciferase with high efficiency and was used as positive control. Correction value = (SRLU-BRLU)/(Sβ-G-Bβ-G); S: recombinant clone sample; B: negative control; RLU (relative light unit): luciferase activity; β-G: β- galactosidase activity. Results are the Mean ± S.D of 3 independent experiments and asterisk symbol indicates P<0.05(*) or P<0.01(**) compared with the control group.