Skip to main content
Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

< Back to Article

Altered Biomarkers of Mucosal Immunity and Reduced Vaginal Lactobacillus Concentrations in Sexually Active Female Adolescents

Figure 1

Anti-HSV (1A) and anti-E. coli activity (1B) for adolescent and adult cervical vaginal lavage (CVL).

For anti-HSV activity, Vero cells were infected with HSV-2(G) mixed 1∶1 with each CVL specimen or control buffer. Viral plaques were counted after 48 hours and percent inhibition calculated relative to control. For E. coli bactericidal activity, 3 µl of stationary phase bacteria (∼109 colony forming units[cfu]/ml) were mixed with 27 µl of each CVL specimen or control buffer and incubated at 37°C for two hours. The mixtures were then further diluted in buffer to yield 800–1000 cfu in control specimens and plated in duplicate on agar enriched with trypticase soy broth. Colonies were counted after an overnight incubation at 37°C and percent inhibition calculated relative to control. All samples were tested in duplicate in two independent experiments. The horizontal lines represent the median value for each group, compared by Mann Whitney U test.

Figure 1

doi: https://doi.org/10.1371/journal.pone.0040415.g001