The Hinge Region of Human Thyroid-Stimulating Hormone (TSH) Receptor Operates as a Tunable Switch between Hormone Binding and Receptor Activation
Figure 4
Epitope mapping of MAb 311.62 by Peptide Phage Display analysis.
A. Amino acid sequences deduced from the phage clones selected after three rounds of biopanning. Three groups of sequence A, B, C were obtained respectively on analyzing the sequence and consensus obtained for each. The numbers in the parentheses denote the frequency or ratio of the number of the phage clones expressing the common peptide sequence to that of the total phage clones. B. Sequence alignment of the consensus sequence derived from the phage clones with the full length TSH receptor revealed sequence similarity in TSH receptor region 266–281. C. Multiple sequence alignment of hTSHR, hLHR and hFSHR corresponding to the region 265–281 of hTSHR. TSHR residues marked in bold were mutated to corresponding residues of LHR. D. MAb 311.62 IgG (25 µg/ml) was pre-incubated with different TSH receptor fragment protein (50 µg/ml) and then added to HEK293-hTSHR cells, and cAMP produced was determined.