A Pro-Cathepsin L Mutant Is a Luminal Substrate for Endoplasmic-Reticulum-Associated Degradation in C. elegans
Figure 6
Autophagy inhibition did not affect steady-state levels of CPL-1W32A;Y35A::YFP.
(A) CPL-1W32A;Y35A::YFP animals were exposed to an autophagy RNAi panel and analyzed as described in Figure 3. Statistical analysis of the RNAi treated animals relative to vector was performed using an unpaired, 2 tailed t-test (unequal variance). No significant difference was observed. (B) VK1879 (N2;vkEx1879[Pnhx-2cpl-1W32AY35A::YFP;Pmyo-2mCherry]) animals were crossed with the autophagy-deficient knockout strain unc-51(e369) to derive unc-51(e369);vkEx1879[Pnhx-2cpl-1W32AY35A::YFP;Pmyo-2mCherry]. Two individual lines (VK1984 and VK1985) were selected and analyzed as described in Figure 3. Results were compared to those obtained in the original CPL-1W32A;Y35A::YFP strain. No significant difference in CPL-1W32A;Y35A::YFP expression was observed in the autophagy deficient strains.