Achieving λ/10 Resolution CW STED Nanoscopy with a Ti:Sapphire Oscillator
Figure 4
Confocal, corresponding CW-STED, and deconvolved STED images of microtubules in a Vero cell stained with Dylight 650.
(a) Confocal; (b) CW-STED; (c) R-L deconvolution of (b). The intensity at the cross-section marked in (a), (b), and (c) inlets are plotted in (d). The wavelength and power density for the STED beam is 783 nm and ∼200 MW/cm2. Scale bar: 1 µm.