Marking and Quantifying IL-17A-Producing Cells In Vivo
Figure 1
(A) Targeting strategy for the il17a locus. For detailed description, see Materials and Methods. (B) CD4+ T cells were isolated from wild-type or Smart-17A mice and polarized under Th17 conditions for 4 days. hNGFR was detected using a surface antibody and IL-17A was assayed using intracellular cytokine staining after restimulation. A representative flow cytometry plot is shown from >5 comparable experiments.