NOTCH1 Signaling Promotes Human T-Cell Acute Lymphoblastic Leukemia Initiating Cell Regeneration in Supportive Niches
Figure 4
hN1 mAb treatment inhibits NOTCH1Mutated LIC burden.
(A) Comparative FACS analysis of human CD34+CD45+ cells and CD34+CD2+ leukemic burden in the bone marrows from NOTCH1Mutated LIC (Patient 11) engrafted mice following treatment with control mAb (left panel) or hN1 mAb (right panel). (B) FACS analysis of human CD34+ and NOTCH1+ cell survival in the mouse spleens following control mAb (n = 9) or hN1 mAb treatment (n = 9) of NOTCH1Mutated LIC (Patients 05, 08, 11) engrafted mice (upper panel). Representative FACS plots show the reduction in both CD34+ and NOTCH1+ cell populations. (C) Representative FACS analysis demonstrating engraftment of CD34+CD45+ cells in the bone marrows of secondary (2°) transplant recipients following transplantation of control mAb (left panel) or hN1 mAb (right panel) treated bone marrow (Patient 11). (D) Graph of percent human T-ALL total CD45+ (blue) and CD34+CD45+ (red) cells in the bone marrows of 2° transplant recipients of control mAb (n = 6) and hN1 mAb (n = 6) treated NOTCH1-driven LIC (Patients 02, 11) (error bars ± SEM; P = 0.16, and P = 0.086, respectively, by Student’s t-test). All results reflect data collected from two independent experiments.