Phenotype Selection Reveals Coevolution of Muscle Glycogen and Protein and PTEN as a Gate Keeper for the Accretion of Muscle Mass in Adult Female Mice
Figure 4
Analysis of signal transduction in muscle lysates from 11, 29 and 54-week female DU6P and Fzt:DU mice.
Western blot identified phosphorylated and total expression of the respective signaling molecule. Specific activation was calculated from the ratios of phosphorylated versus total protein. Coomassie blue staining of the membranes used for Western immuno detection was used as loading control. A: IGF-1Rß in membrane fractions (n = 3); B: ILK (n = 9); C: GDF-8, 26 kDa band (n = 11) and D: PTEN (n = 9). The inserts provide representative experiments, whereby all samples were studied on the identical membrane. Sample numbers (n) depict the number of samples per age group, the error bars represent SEM.