Persistence Length of Human Cardiac α-Tropomyosin Measured by Single Molecule Direct Probe Microscopy
Figure 5
End-to-end length analysis shows Lp of WT human cardiac αTm consistent with tangent angle correlation analysis.
Normalized end-to-end length (le-e) distributions from one of the WT αTm samples incubated on p-Lys coated mica substrate for 600 s (N = 798) fits to the WLC model (Eq. 3). lp value from the fit was 1.0425±0.0505 (R2 = 0.88). Errors were estimated by the jackknife method (Materials and Methods).