Broader Neutralizing Antibodies against H5N1 Viruses Using Prime-Boost Immunization of Hyperglycosylated Hemagglutinin DNA and Virus-Like Particles
Figure 7
Total anti-HA IgG titers elicited by hyperglycosylated DNA vaccine priming followed by FliC-VLP boosting.
Mice were immunized with each immunorefocusing HA vector twice followed with a third boosting dose with FliC-VLPs on a three-week interval. Sera were collected at 2 weeks after final immunization, and determined the anti-HA IgG titers by ELISA. The results showed that no significant differences of the HA-specific total IgG titers of all the immunized groups with the hyperglycosyalted HA DNA vaccines compared to the wild-type control. Individual titers (points) and geometric means (lines) are given for each group.