Regional Susceptibility to TNF-α Induction of Murine Brain Inflammation via Classical IKK/NF-κB Signalling
Figure 1
Immunofluorescent staining of primary cortical neurons.
A: p65 expression in primary cortical neurons, showing increased expression with TNF-α and decreased expression with IKK Inhibitor (F = 177.79; p<0.001; df = 2,12). B: NF-κ B expression in a control neuron, using anti-p65 antibody and FITC labelled secondary, NF-κ B expression in a TNF-α stimulated neuron and NF-κ B expression in a TNF-α stimulated neuron with NF-κ B inhibitor. C: The percentage of neurons with anti-p65 staining in the nucleus (F = 30.63; p<0.001; df = 2,14), and D: the levels of fluorescence in each sample where fluorescence is rated between 0 and 3 on the scale bar (F = 17.65; p<0.002; df = 2,57). Molecular weight markers are in kilodaltons (kDa).