Med5(Nut1) and Med17(Srb4) Are Direct Targets of Mediator Histone H4 Tail Interactions
Figure 5
The C-terminus of Med14(Rgr1)p does not directly contribute to H4-Mediator interaction.
(A) Immunoblotting analysis comparing the composition of affinity-purified rgr1(Med14)-Δ2 and WT Mediator complexes after 10% SDS-PAGE. (B) Western blot analysis of histone tail binding experiment comparing the H4 tail binding affinity of WT, Δmed5(nut1) and rgr1(Med14)-Δ2 Mediator complexes. An equal concentration (∼6 nM) of WT, Δmed5(nut1) or rgr1(Med14)-Δ2 Mediator complex was mixed with WT H4 peptide (1.4 µM) as the inputs. The basic steps and layout of the analysis were as described earlier (Fig. 1).