Isolation of HIV-1-Neutralizing Mucosal Monoclonal Antibodies from Human Colostrum
Figure 3
ADCC and virus capture by mAbs CH07 and CH08.
A. CH07 and CH08 were tested for ADCC activity compared with anti-RSV mAb (Palivizumab) and A32. CH07 did not mediate ADCC, while both A32 and CH08 stimulated granzyme B activity NK effector cells when reacted with infected and gp120-coated targets. Each bar represents the percent of target cells containing granzyme B. B. CH07 did not capture pseudoviruses SF162.B, BG1168.B, and CAP45.C. Data shown as p24 released from captured virus. C. CH08 captured all three pseudoviruses in the presence of sCD4 but not when sCD4 was absent. Data shown as p24 released from captured virus.