Extracellular Matrix Mineralization Promotes E11/gp38 Glycoprotein Expression and Drives Osteocytic Differentiation
Figure 7
Effect of reversal of ECM mineralization inhibition on MLO-A5 cells.
(A) Alizarin red staining of MLO-A5 cultures in which mineralization was inhibited for 6 days before being promoted for a further 9 days. (B) Quantification of the results from (A) by spectrometry. Results are mean±SEM (n = 3). (C) E11 protein expression under the same conditions as (A) and (B). (D) Densitometric analysis of 3 independent E11 western blots. Results are mean±SEM (n = 3). *P<0.05, **P<0.01, ***P<0.001 compared to previous time-point.