Activation of P2X7 Receptor by ATP Plays an Important Role in Regulating Inflammatory Responses during Acute Viral Infection
Figure 1
Treatment with oATP inhibited inflammatory responses in Ad infected MLE-Raw co-culture.
The MLE-Raw co-culture was infected with Ad for 24 h in the presence of oATP at the designated concentrations. (A), The amount of NO produced in the culture was measured from the medium using Griess reagent. Data are expressed as mean ± SD. (B), The MLE-Raw co-culture was infected with Ad and ROS positive cells from were counted by flow cytometry after treating with 3-(p-aminophenyl) fluorescein (APF) without gating for specific cell types. Shown is a representative FACS histogram of three independent experiments with the percentage of ROS positive population. (C and D) Mouse IL-6 and KC, respectively, were measured from the medium using commercially available ELISA kits. Data are expressed as mean ± standard deviation (SD).